Fig. 1. Acid ceramidase treatment does not induce cell death or proliferation. Chang (A, C) or Jurkat (B, D) cells were treated with acid ceramidase for the indicated times and cell death was determined by trypan blue staining. Cell proliferation was measured by counting the cells. Treatment with the known death stimuli 1 mM staurosporine or 10 mg/ml anti-CD95 antibodies CH11 served as positive control. Given are the mean ± SD from 4 independent studies, ***p<0.001, ANOVA.